annexin v fitc pi apoptosis detection kit (Elabscience Biotechnology)
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Annexin V Fitc Pi Apoptosis Detection Kit, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/annexin+v+pe/Annexin+V-PE%2F7-AAD+Apoptosis+Kit/10__1186_slash_s13023___026___04548___3-107-10-15
Average 94 stars, based on 36 article reviews
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Binding Assay:Article Title: SDF2 promotes glioma progression via GRP78‑mediated ERAD and copper homeostasis disruption. Article Snippet: .. The cells were then resuspended in binding buffer from an Article Title: NDUFAB1 promotes hepatocellular carcinoma progression by disrupting mitochondrial homeostasis and mitophagy pathway. Article Snippet: NADH: ubiquinone oxidoreductase subunit AB1 (NDUFAB1), a critical factor of mitochondrial complex I in electron transport chain, is essential for cellular energy metabolism; however, its specific functions and mechanisms in hepatocellular carcinoma (HCC) remain elusive.. We found significantly elevated NDUFAB1 expression in HCC tissues, which is correlated significantly with poor patient prognosis and may serve as an independent risk factor (P < 0.05).. Loss-of-function experiments revealed that NDUFAB1 knockdown reduced HCC cell proliferation, metastatic potential, and invasive capacity while promoting apoptosis. Article Title: The HOTAIRM1-miR-222 Axis Regulates Venetoclax Resistance and Defines a High-Risk Subset in Pediatric t(8;21) Acute Myeloid Leukemia Article Snippet: Cell cycle and apoptosis were assessed using a BD FACS MelodyTM flow cytometer (BD Biosciences, San Jose, CA, USA). .. AML cell lines (Kasumi-1, MOLM-13, THP-1) were treated, fixed in 70% ethanol at 4°C for 30 min, and stained with 50 μg/mL 7-AAD (BD Biosciences) following incubation in binding buffer (0.1% Triton X-100, 0.1% citrate in PBS) at 37°C for 30 min. For apoptosis analysis, cells were stained with 2.5 μL each of Article Title: Identification and regulatory mechanisms of biomarkers related to endoplasmic reticulum stress in coronary artery disease Article Snippet: .. A bar chart was created using Prism software, and a bar chart was made using the target gray value. (c)Cell survival rate: After adding 10 μL of CCK solution to groups B and C and incubating, the absorbance was measured with a microplate reader. (d) Apoptosis (Annexin V/PI flow cytometry): 50 μL of 1 × Binding Buffer working solution, 5 μL of Article Title: SDF2 promotes glioma progression via GRP78-mediated ERAD and copper homeostasis disruption Article Snippet: .. The cells were then resuspended in binding buffer from an Incubation:Article Title: SDF2 promotes glioma progression via GRP78‑mediated ERAD and copper homeostasis disruption. Article Snippet: .. The cells were then resuspended in binding buffer from an Article Title: The HOTAIRM1-miR-222 Axis Regulates Venetoclax Resistance and Defines a High-Risk Subset in Pediatric t(8;21) Acute Myeloid Leukemia Article Snippet: Cell cycle and apoptosis were assessed using a BD FACS MelodyTM flow cytometer (BD Biosciences, San Jose, CA, USA). .. AML cell lines (Kasumi-1, MOLM-13, THP-1) were treated, fixed in 70% ethanol at 4°C for 30 min, and stained with 50 μg/mL 7-AAD (BD Biosciences) following incubation in binding buffer (0.1% Triton X-100, 0.1% citrate in PBS) at 37°C for 30 min. For apoptosis analysis, cells were stained with 2.5 μL each of Article Title: SDF2 promotes glioma progression via GRP78-mediated ERAD and copper homeostasis disruption Article Snippet: .. The cells were then resuspended in binding buffer from an Staining:Article Title: NDUFAB1 promotes hepatocellular carcinoma progression by disrupting mitochondrial homeostasis and mitophagy pathway. Article Snippet: NADH: ubiquinone oxidoreductase subunit AB1 (NDUFAB1), a critical factor of mitochondrial complex I in electron transport chain, is essential for cellular energy metabolism; however, its specific functions and mechanisms in hepatocellular carcinoma (HCC) remain elusive.. We found significantly elevated NDUFAB1 expression in HCC tissues, which is correlated significantly with poor patient prognosis and may serve as an independent risk factor (P < 0.05).. Loss-of-function experiments revealed that NDUFAB1 knockdown reduced HCC cell proliferation, metastatic potential, and invasive capacity while promoting apoptosis. Article Title: The HOTAIRM1-miR-222 Axis Regulates Venetoclax Resistance and Defines a High-Risk Subset in Pediatric t(8;21) Acute Myeloid Leukemia Article Snippet: Cell cycle and apoptosis were assessed using a BD FACS MelodyTM flow cytometer (BD Biosciences, San Jose, CA, USA). .. AML cell lines (Kasumi-1, MOLM-13, THP-1) were treated, fixed in 70% ethanol at 4°C for 30 min, and stained with 50 μg/mL 7-AAD (BD Biosciences) following incubation in binding buffer (0.1% Triton X-100, 0.1% citrate in PBS) at 37°C for 30 min. For apoptosis analysis, cells were stained with 2.5 μL each of Article Title: Inhibition of replication factor C4-induced DNA damage repair reverses the chemoresistance of glioblastoma to temozolomide and reveals the synthetic lethal effect of combined targeting of checkpoint kinase 1. Article Snippet: Glioblastoma (GBM) is a highly aggressive malignant tumor of the central nervous system, and resistance to temozolomide (TMZ) remains a major cause of treatment failure and tumor recurrence.. DNA damage repair (DDR) serves as a critical mechanism underlying chemoresistance, with replication factor C4 (RFC4) emerging as a key mediator.. We aimed to investigate the mechanism by which RFC4 induces TMZ resistance in GBM through DDR activation. Article Title: Psoralidin induces pyroptosis in both tumor cells and macrophages as well as enhances nature killer cell cytotoxicity to suppress hepatocellular carcinoma. Article Snippet: Psoralidin is a major component of the traditional Chinese medicine Psoraleae Fructus, which is derived from the dried mature fruit of the leguminous plant Psoralea corylifolia L. and possesses many pharmacological effects, including anti-tumor effects.. However, the mechanism through which psoralidin protects against hepatocellular carcinoma (HCC) remains unclear.. In our study, we found that psoralidin induced pyroptosis and gasdermin E (GSDME) cleavage in HepG2 and Hepa1–6 cells, which were reversed by the caspase-3 inhibitor Z-DEVD-FMK. Software:Article Title: Identification and regulatory mechanisms of biomarkers related to endoplasmic reticulum stress in coronary artery disease Article Snippet: .. A bar chart was created using Prism software, and a bar chart was made using the target gray value. (c)Cell survival rate: After adding 10 μL of CCK solution to groups B and C and incubating, the absorbance was measured with a microplate reader. (d) Apoptosis (Annexin V/PI flow cytometry): 50 μL of 1 × Binding Buffer working solution, 5 μL of Flow Cytometry:Article Title: Identification and regulatory mechanisms of biomarkers related to endoplasmic reticulum stress in coronary artery disease Article Snippet: .. A bar chart was created using Prism software, and a bar chart was made using the target gray value. (c)Cell survival rate: After adding 10 μL of CCK solution to groups B and C and incubating, the absorbance was measured with a microplate reader. (d) Apoptosis (Annexin V/PI flow cytometry): 50 μL of 1 × Binding Buffer working solution, 5 μL of Double Staining:Article Title: Neuroprotection effect of HSV-1 LAT-derived miR-H2 and miR-H3 associated with Tau and alpha-synuclein downregulation. Article Snippet: .. Apoptotic cell death was further evaluated using the |
