Review



annexin v fitc pi apoptosis detection kit  (Elabscience Biotechnology)


Bioz Verified Symbol Elabscience Biotechnology is a verified supplier
Bioz Manufacturer Symbol Elabscience Biotechnology manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    Elabscience Biotechnology annexin v fitc pi apoptosis detection kit
    Annexin V Fitc Pi Apoptosis Detection Kit, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/annexin+v+pe/Annexin+V-PE%2F7-AAD+Apoptosis+Kit/10__1186_slash_s13023___026___04548___3-107-10-15
    Average 94 stars, based on 36 article reviews
    annexin v fitc pi apoptosis detection kit - by Bioz Stars, 2026-10
    94/100 stars

    Images

    Related Articles

    Binding Assay:

    Article Title: SDF2 promotes glioma progression via GRP78‑mediated ERAD and copper homeostasis disruption.
    Article Snippet: .. The cells were then resuspended in binding buffer from an Annexin V‐PE/7‐AAD Apoptosis Detection Kit (cat. no. E‐CK‐A216; Elabscience Biotechnology, Inc.) and incubated in the dark at room temperature for 20 min. .. Following incubation, the cells were washed, resuspended and analyzed for apoptosis by flow cytometry (CytoFLEX; Beckman Coulter, Inc.).

    Article Title: NDUFAB1 promotes hepatocellular carcinoma progression by disrupting mitochondrial homeostasis and mitophagy pathway.
    Article Snippet: NADH: ubiquinone oxidoreductase subunit AB1 (NDUFAB1), a critical factor of mitochondrial complex I in electron transport chain, is essential for cellular energy metabolism; however, its specific functions and mechanisms in hepatocellular carcinoma (HCC) remain elusive.. We found significantly elevated NDUFAB1 expression in HCC tissues, which is correlated significantly with poor patient prognosis and may serve as an independent risk factor (P < 0.05).. Loss-of-function experiments revealed that NDUFAB1 knockdown reduced HCC cell proliferation, metastatic potential, and invasive capacity while promoting apoptosis.

    Article Title: The HOTAIRM1-miR-222 Axis Regulates Venetoclax Resistance and Defines a High-Risk Subset in Pediatric t(8;21) Acute Myeloid Leukemia
    Article Snippet: Cell cycle and apoptosis were assessed using a BD FACS MelodyTM flow cytometer (BD Biosciences, San Jose, CA, USA). .. AML cell lines (Kasumi-1, MOLM-13, THP-1) were treated, fixed in 70% ethanol at 4°C for 30 min, and stained with 50 μg/mL 7-AAD (BD Biosciences) following incubation in binding buffer (0.1% Triton X-100, 0.1% citrate in PBS) at 37°C for 30 min. For apoptosis analysis, cells were stained with 2.5 μL each of Annexin V-PE and 7-AAD using the 7-AAD/Annexin V-FITC Apoptosis Detection Kit (Elabscience, Houston, TX, USA) and incubated in the dark for 15 min. .. Samples were acquired on the BD FACS MelodyTM and analyzed using FlowJo v10.8 (BD Biosciences).

    Article Title: Identification and regulatory mechanisms of biomarkers related to endoplasmic reticulum stress in coronary artery disease
    Article Snippet: .. A bar chart was created using Prism software, and a bar chart was made using the target gray value. (c)Cell survival rate: After adding 10 μL of CCK solution to groups B and C and incubating, the absorbance was measured with a microplate reader. (d) Apoptosis (Annexin V/PI flow cytometry): 50 μL of 1 × Binding Buffer working solution, 5 μL of Annexin V-PE (lab science, E-CK-A216), and 5 μL of 7-AAD (elabscience, E-CK-A216) were successively added to the cell precipitation. ..

    Article Title: SDF2 promotes glioma progression via GRP78-mediated ERAD and copper homeostasis disruption
    Article Snippet: .. The cells were then resuspended in binding buffer from an Annexin V-PE/7-AAD Apoptosis Detection Kit (cat. no. E-CK-A216; Elabscience Biotechnology, Inc.) and incubated in the dark at room temperature for 20 min. .. Following incubation, the cells were washed, resuspended and analyzed for apoptosis by flow cytometry (CytoFLEX; Beckman Coulter, Inc.).

    Incubation:

    Article Title: SDF2 promotes glioma progression via GRP78‑mediated ERAD and copper homeostasis disruption.
    Article Snippet: .. The cells were then resuspended in binding buffer from an Annexin V‐PE/7‐AAD Apoptosis Detection Kit (cat. no. E‐CK‐A216; Elabscience Biotechnology, Inc.) and incubated in the dark at room temperature for 20 min. .. Following incubation, the cells were washed, resuspended and analyzed for apoptosis by flow cytometry (CytoFLEX; Beckman Coulter, Inc.).

    Article Title: The HOTAIRM1-miR-222 Axis Regulates Venetoclax Resistance and Defines a High-Risk Subset in Pediatric t(8;21) Acute Myeloid Leukemia
    Article Snippet: Cell cycle and apoptosis were assessed using a BD FACS MelodyTM flow cytometer (BD Biosciences, San Jose, CA, USA). .. AML cell lines (Kasumi-1, MOLM-13, THP-1) were treated, fixed in 70% ethanol at 4°C for 30 min, and stained with 50 μg/mL 7-AAD (BD Biosciences) following incubation in binding buffer (0.1% Triton X-100, 0.1% citrate in PBS) at 37°C for 30 min. For apoptosis analysis, cells were stained with 2.5 μL each of Annexin V-PE and 7-AAD using the 7-AAD/Annexin V-FITC Apoptosis Detection Kit (Elabscience, Houston, TX, USA) and incubated in the dark for 15 min. .. Samples were acquired on the BD FACS MelodyTM and analyzed using FlowJo v10.8 (BD Biosciences).

    Article Title: SDF2 promotes glioma progression via GRP78-mediated ERAD and copper homeostasis disruption
    Article Snippet: .. The cells were then resuspended in binding buffer from an Annexin V-PE/7-AAD Apoptosis Detection Kit (cat. no. E-CK-A216; Elabscience Biotechnology, Inc.) and incubated in the dark at room temperature for 20 min. .. Following incubation, the cells were washed, resuspended and analyzed for apoptosis by flow cytometry (CytoFLEX; Beckman Coulter, Inc.).

    Staining:

    Article Title: NDUFAB1 promotes hepatocellular carcinoma progression by disrupting mitochondrial homeostasis and mitophagy pathway.
    Article Snippet: NADH: ubiquinone oxidoreductase subunit AB1 (NDUFAB1), a critical factor of mitochondrial complex I in electron transport chain, is essential for cellular energy metabolism; however, its specific functions and mechanisms in hepatocellular carcinoma (HCC) remain elusive.. We found significantly elevated NDUFAB1 expression in HCC tissues, which is correlated significantly with poor patient prognosis and may serve as an independent risk factor (P < 0.05).. Loss-of-function experiments revealed that NDUFAB1 knockdown reduced HCC cell proliferation, metastatic potential, and invasive capacity while promoting apoptosis.

    Article Title: The HOTAIRM1-miR-222 Axis Regulates Venetoclax Resistance and Defines a High-Risk Subset in Pediatric t(8;21) Acute Myeloid Leukemia
    Article Snippet: Cell cycle and apoptosis were assessed using a BD FACS MelodyTM flow cytometer (BD Biosciences, San Jose, CA, USA). .. AML cell lines (Kasumi-1, MOLM-13, THP-1) were treated, fixed in 70% ethanol at 4°C for 30 min, and stained with 50 μg/mL 7-AAD (BD Biosciences) following incubation in binding buffer (0.1% Triton X-100, 0.1% citrate in PBS) at 37°C for 30 min. For apoptosis analysis, cells were stained with 2.5 μL each of Annexin V-PE and 7-AAD using the 7-AAD/Annexin V-FITC Apoptosis Detection Kit (Elabscience, Houston, TX, USA) and incubated in the dark for 15 min. .. Samples were acquired on the BD FACS MelodyTM and analyzed using FlowJo v10.8 (BD Biosciences).

    Article Title: Inhibition of replication factor C4-induced DNA damage repair reverses the chemoresistance of glioblastoma to temozolomide and reveals the synthetic lethal effect of combined targeting of checkpoint kinase 1.
    Article Snippet: Glioblastoma (GBM) is a highly aggressive malignant tumor of the central nervous system, and resistance to temozolomide (TMZ) remains a major cause of treatment failure and tumor recurrence.. DNA damage repair (DDR) serves as a critical mechanism underlying chemoresistance, with replication factor C4 (RFC4) emerging as a key mediator.. We aimed to investigate the mechanism by which RFC4 induces TMZ resistance in GBM through DDR activation.

    Article Title: Psoralidin induces pyroptosis in both tumor cells and macrophages as well as enhances nature killer cell cytotoxicity to suppress hepatocellular carcinoma.
    Article Snippet: Psoralidin is a major component of the traditional Chinese medicine Psoraleae Fructus, which is derived from the dried mature fruit of the leguminous plant Psoralea corylifolia L. and possesses many pharmacological effects, including anti-tumor effects.. However, the mechanism through which psoralidin protects against hepatocellular carcinoma (HCC) remains unclear.. In our study, we found that psoralidin induced pyroptosis and gasdermin E (GSDME) cleavage in HepG2 and Hepa1–6 cells, which were reversed by the caspase-3 inhibitor Z-DEVD-FMK.

    Software:

    Article Title: Identification and regulatory mechanisms of biomarkers related to endoplasmic reticulum stress in coronary artery disease
    Article Snippet: .. A bar chart was created using Prism software, and a bar chart was made using the target gray value. (c)Cell survival rate: After adding 10 μL of CCK solution to groups B and C and incubating, the absorbance was measured with a microplate reader. (d) Apoptosis (Annexin V/PI flow cytometry): 50 μL of 1 × Binding Buffer working solution, 5 μL of Annexin V-PE (lab science, E-CK-A216), and 5 μL of 7-AAD (elabscience, E-CK-A216) were successively added to the cell precipitation. ..

    Flow Cytometry:

    Article Title: Identification and regulatory mechanisms of biomarkers related to endoplasmic reticulum stress in coronary artery disease
    Article Snippet: .. A bar chart was created using Prism software, and a bar chart was made using the target gray value. (c)Cell survival rate: After adding 10 μL of CCK solution to groups B and C and incubating, the absorbance was measured with a microplate reader. (d) Apoptosis (Annexin V/PI flow cytometry): 50 μL of 1 × Binding Buffer working solution, 5 μL of Annexin V-PE (lab science, E-CK-A216), and 5 μL of 7-AAD (elabscience, E-CK-A216) were successively added to the cell precipitation. ..

    Double Staining:

    Article Title: Neuroprotection effect of HSV-1 LAT-derived miR-H2 and miR-H3 associated with Tau and alpha-synuclein downregulation.
    Article Snippet: .. Apoptotic cell death was further evaluated using the Annexin V PE/7-AAD double staining apoptosis detection kit (Elabscience, USA). .. Following 21 days of hUCS treatment, apoptosis rates were quantified by flow cytometry (BD Biosciences, San Jose, CA, USA), with data analysis performed using FlowJo software (version 7.6.5; Tree Star, Inc., Ashland, OR, USA).



    Similar Products

    96
    Vazyme Biotech Co flow cytometry
    Flow Cytometry, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/annexin+v+pe/Annexin+V-PE+7-AAD+Apoptosis+Detection+Kit/pm42537352-103-4-7
    Average 96 stars, based on 1 article reviews
    flow cytometry - by Bioz Stars, 2026-10
    96/100 stars
      Buy from Supplier

    95
    Multi Sciences (Lianke) Biotech Co Ltd accutase
    Accutase, supplied by Multi Sciences (Lianke) Biotech Co Ltd, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/annexin+v+pe/Annexin+V-PE%2F7-AAD+Apoptosis+Kit/pm42425028-141-18-27
    Average 95 stars, based on 1 article reviews
    accutase - by Bioz Stars, 2026-10
    95/100 stars
      Buy from Supplier

    97
    Beyotime annexin v pe
    Annexin V Pe, supplied by Beyotime, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/annexin+v+pe/Annexin+V-PE+Apoptosis+Detection+Kit/pm42803824-106-25-30
    Average 97 stars, based on 1 article reviews
    annexin v pe - by Bioz Stars, 2026-10
    97/100 stars
      Buy from Supplier

    97
    Beyotime annexin v fitc pi apoptosis detection kit
    Annexin V Fitc Pi Apoptosis Detection Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/annexin+v+pe/Annexin+V-PE+Apoptosis+Detection+Kit/pmc13085010-48-4-17
    Average 97 stars, based on 1 article reviews
    annexin v fitc pi apoptosis detection kit - by Bioz Stars, 2026-10
    97/100 stars
      Buy from Supplier

    94
    Elabscience Biotechnology annexin v fitc pi apoptosis detection kit
    Annexin V Fitc Pi Apoptosis Detection Kit, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/annexin+v+pe/Annexin+V-PE%2F7-AAD+Apoptosis+Kit/10__1186_slash_s13023___026___04548___3-107-10-15
    Average 94 stars, based on 1 article reviews
    annexin v fitc pi apoptosis detection kit - by Bioz Stars, 2026-10
    94/100 stars
      Buy from Supplier

    94
    MedChemExpress p re ss pi pe apoptosis detection kit
    P Re Ss Pi Pe Apoptosis Detection Kit, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/annexin+v+pe/Annexin+V-PE+Apoptosis+Detection+Kit/pm42342666-398-17-24
    Average 94 stars, based on 1 article reviews
    p re ss pi pe apoptosis detection kit - by Bioz Stars, 2026-10
    94/100 stars
      Buy from Supplier

    95
    MedChemExpress annexin v fitc propidium iodide pi pe apoptosis detection kit
    A Flow cytometry analysis of neutrophil <t>apoptosis</t> after coculture with sodium decanoate (DeaNa), sodium palmitate (PaNa), and PBS. Quantitative graphs showing the proportion of late apoptotic/necrotic <t>(Annexin</t> V⁺/PI⁺) neutrophils ( B ) and early apoptotic (Annexin V⁺/PI⁻) neutrophils ( C ) under different treatments ( n = 3). D Schematic representation of neutrophil migration in vitro for 3 h at 37 °C. Created in BioRender. Yang, Q. (2026) https://BioRender.com/fm3t1j5 . Representative images of migrated neutrophils on the lower membrane stained with DAPI and photographed using the multifunctional enzyme reader. E Numbers of migrating neutrophils ( n = 3). F Volcano plot of differential gene expression in sodium decanoate-treated and PBS-treated neutrophils. G Heatmap showing the DEGs identified via RNA-seq in the presence of decanoic acid or PBS. H Quantitative reverse transcription PCR (qRT‒PCR) validation of gene expression changes in neutrophils treated with decanoic acid, palmitic acid and PBS ( n = 4). I Cell supernatant CXCL8 expression in neutrophils treated with decanoic acid, palmitic acid and PBS, as determined by ELISA ( n = 4). J Simplified schematic illustrating the coculture assay of neutrophils with Caco-2 cells, detailing the experimental setup. Created in BioRender. Yang, Q. (2026) https://BioRender.com/74ys0y4 . K qRT‒PCR validation of gene expression changes in Caco-2 cells after coculture with different fatty-acid-treated neutrophils ( n = 3). Data are shown as mean ± SEM, with representative results from at least three independent experiments. P values were determined by one-way ANOVA with Holm–Šidák test ( B , C ), one-way ANOVA with Dunnett’s test ( E , K ), one-way ANOVA with Tukey test ( H – I ). DeaNa, sodium decanoate; PaNa, sodium palmitate. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 and ns P > 0.05. Exact P values: B DeaNa vs PBS, P = 0.0465, PaNa vs PBS, P = 0.0002; C DeaNa vs PBS, P < 0.0001, PaNa vs PBS, P < 0.0001; E DeaNa vs PBS, P = 0.0342, PaNa vs PBS, P = 0.4726; H Cxcl2 , DeaNa vs PBS, P = 0.0015, PaNa vs PBS, P = 0.0001; Cxcl8 , DeaNa vs PBS, P = 0.0120, PaNa vs PBS, P = 0.0725; Cpt1a , DeaNa vs PBS, P = 0.0441, PaNa vs PBS, P < 0.0001; Osm , DeaNa vs PBS, P = 0.0078, PaNa vs PBS, P = 0.0019; Vegfa , DeaNa vs PBS, P = 0.0319, PaNa vs PBS, P = 0.0009. I DeaNa vs PBS, P = 0.0016, PaNa vs PBS, P = 0.0101. K CXCL8, DeaNa vs PBS, P < 0.0001, PaNa vs PBS, P = 0.9138; OSM, DeaNa vs PBS, P = 0.0865, PaNa vs PBS, P = 0.0031; VEGFA, DeaNa vs PBS, P = 0.0053, PaNa vs PBS, P < 0.0001; AREG, DeaNa vs PBS, P = 0.0451, PaNa vs PBS, P = 0.0391; PTGS2, DeaNa vs PBS, P = 0.0015, PaNa vs PBS, P = 0.0224.
    Annexin V Fitc Propidium Iodide Pi Pe Apoptosis Detection Kit, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/annexin+v+pe/Annexin+A5%2FANXA5%2C+Human/pmc13443604-502-9-16
    Average 95 stars, based on 1 article reviews
    annexin v fitc propidium iodide pi pe apoptosis detection kit - by Bioz Stars, 2026-10
    95/100 stars
      Buy from Supplier

    86
    Yeasen Biotechnology annexin v fitc pi pe apoptosis detection kit
    A Flow cytometry analysis of neutrophil <t>apoptosis</t> after coculture with sodium decanoate (DeaNa), sodium palmitate (PaNa), and PBS. Quantitative graphs showing the proportion of late apoptotic/necrotic <t>(Annexin</t> V⁺/PI⁺) neutrophils ( B ) and early apoptotic (Annexin V⁺/PI⁻) neutrophils ( C ) under different treatments ( n = 3). D Schematic representation of neutrophil migration in vitro for 3 h at 37 °C. Created in BioRender. Yang, Q. (2026) https://BioRender.com/fm3t1j5 . Representative images of migrated neutrophils on the lower membrane stained with DAPI and photographed using the multifunctional enzyme reader. E Numbers of migrating neutrophils ( n = 3). F Volcano plot of differential gene expression in sodium decanoate-treated and PBS-treated neutrophils. G Heatmap showing the DEGs identified via RNA-seq in the presence of decanoic acid or PBS. H Quantitative reverse transcription PCR (qRT‒PCR) validation of gene expression changes in neutrophils treated with decanoic acid, palmitic acid and PBS ( n = 4). I Cell supernatant CXCL8 expression in neutrophils treated with decanoic acid, palmitic acid and PBS, as determined by ELISA ( n = 4). J Simplified schematic illustrating the coculture assay of neutrophils with Caco-2 cells, detailing the experimental setup. Created in BioRender. Yang, Q. (2026) https://BioRender.com/74ys0y4 . K qRT‒PCR validation of gene expression changes in Caco-2 cells after coculture with different fatty-acid-treated neutrophils ( n = 3). Data are shown as mean ± SEM, with representative results from at least three independent experiments. P values were determined by one-way ANOVA with Holm–Šidák test ( B , C ), one-way ANOVA with Dunnett’s test ( E , K ), one-way ANOVA with Tukey test ( H – I ). DeaNa, sodium decanoate; PaNa, sodium palmitate. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 and ns P > 0.05. Exact P values: B DeaNa vs PBS, P = 0.0465, PaNa vs PBS, P = 0.0002; C DeaNa vs PBS, P < 0.0001, PaNa vs PBS, P < 0.0001; E DeaNa vs PBS, P = 0.0342, PaNa vs PBS, P = 0.4726; H Cxcl2 , DeaNa vs PBS, P = 0.0015, PaNa vs PBS, P = 0.0001; Cxcl8 , DeaNa vs PBS, P = 0.0120, PaNa vs PBS, P = 0.0725; Cpt1a , DeaNa vs PBS, P = 0.0441, PaNa vs PBS, P < 0.0001; Osm , DeaNa vs PBS, P = 0.0078, PaNa vs PBS, P = 0.0019; Vegfa , DeaNa vs PBS, P = 0.0319, PaNa vs PBS, P = 0.0009. I DeaNa vs PBS, P = 0.0016, PaNa vs PBS, P = 0.0101. K CXCL8, DeaNa vs PBS, P < 0.0001, PaNa vs PBS, P = 0.9138; OSM, DeaNa vs PBS, P = 0.0865, PaNa vs PBS, P = 0.0031; VEGFA, DeaNa vs PBS, P = 0.0053, PaNa vs PBS, P < 0.0001; AREG, DeaNa vs PBS, P = 0.0451, PaNa vs PBS, P = 0.0391; PTGS2, DeaNa vs PBS, P = 0.0015, PaNa vs PBS, P = 0.0224.
    Annexin V Fitc Pi Pe Apoptosis Detection Kit, supplied by Yeasen Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/annexin+v+pe/annexin+apoptosis+detection+fitc+kit+pi+v/pm42304385-87-5-13
    Average 86 stars, based on 1 article reviews
    annexin v fitc pi pe apoptosis detection kit - by Bioz Stars, 2026-10
    86/100 stars
      Buy from Supplier

    96
    Vazyme Biotech Co annexin v-pe 7-aad apoptosis detection kit
    A Flow cytometry analysis of neutrophil <t>apoptosis</t> after coculture with sodium decanoate (DeaNa), sodium palmitate (PaNa), and PBS. Quantitative graphs showing the proportion of late apoptotic/necrotic <t>(Annexin</t> V⁺/PI⁺) neutrophils ( B ) and early apoptotic (Annexin V⁺/PI⁻) neutrophils ( C ) under different treatments ( n = 3). D Schematic representation of neutrophil migration in vitro for 3 h at 37 °C. Created in BioRender. Yang, Q. (2026) https://BioRender.com/fm3t1j5 . Representative images of migrated neutrophils on the lower membrane stained with DAPI and photographed using the multifunctional enzyme reader. E Numbers of migrating neutrophils ( n = 3). F Volcano plot of differential gene expression in sodium decanoate-treated and PBS-treated neutrophils. G Heatmap showing the DEGs identified via RNA-seq in the presence of decanoic acid or PBS. H Quantitative reverse transcription PCR (qRT‒PCR) validation of gene expression changes in neutrophils treated with decanoic acid, palmitic acid and PBS ( n = 4). I Cell supernatant CXCL8 expression in neutrophils treated with decanoic acid, palmitic acid and PBS, as determined by ELISA ( n = 4). J Simplified schematic illustrating the coculture assay of neutrophils with Caco-2 cells, detailing the experimental setup. Created in BioRender. Yang, Q. (2026) https://BioRender.com/74ys0y4 . K qRT‒PCR validation of gene expression changes in Caco-2 cells after coculture with different fatty-acid-treated neutrophils ( n = 3). Data are shown as mean ± SEM, with representative results from at least three independent experiments. P values were determined by one-way ANOVA with Holm–Šidák test ( B , C ), one-way ANOVA with Dunnett’s test ( E , K ), one-way ANOVA with Tukey test ( H – I ). DeaNa, sodium decanoate; PaNa, sodium palmitate. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 and ns P > 0.05. Exact P values: B DeaNa vs PBS, P = 0.0465, PaNa vs PBS, P = 0.0002; C DeaNa vs PBS, P < 0.0001, PaNa vs PBS, P < 0.0001; E DeaNa vs PBS, P = 0.0342, PaNa vs PBS, P = 0.4726; H Cxcl2 , DeaNa vs PBS, P = 0.0015, PaNa vs PBS, P = 0.0001; Cxcl8 , DeaNa vs PBS, P = 0.0120, PaNa vs PBS, P = 0.0725; Cpt1a , DeaNa vs PBS, P = 0.0441, PaNa vs PBS, P < 0.0001; Osm , DeaNa vs PBS, P = 0.0078, PaNa vs PBS, P = 0.0019; Vegfa , DeaNa vs PBS, P = 0.0319, PaNa vs PBS, P = 0.0009. I DeaNa vs PBS, P = 0.0016, PaNa vs PBS, P = 0.0101. K CXCL8, DeaNa vs PBS, P < 0.0001, PaNa vs PBS, P = 0.9138; OSM, DeaNa vs PBS, P = 0.0865, PaNa vs PBS, P = 0.0031; VEGFA, DeaNa vs PBS, P = 0.0053, PaNa vs PBS, P < 0.0001; AREG, DeaNa vs PBS, P = 0.0451, PaNa vs PBS, P = 0.0391; PTGS2, DeaNa vs PBS, P = 0.0015, PaNa vs PBS, P = 0.0224.
    Annexin V Pe 7 Aad Apoptosis Detection Kit, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/annexin+v+pe/Annexin+V-PE+7-AAD+Apoptosis+Detection+Kit/custom%40a213%4042269606
    Average 96 stars, based on 1 article reviews
    annexin v-pe 7-aad apoptosis detection kit - by Bioz Stars, 2026-10
    96/100 stars
      Buy from Supplier

    Image Search Results


    A Flow cytometry analysis of neutrophil apoptosis after coculture with sodium decanoate (DeaNa), sodium palmitate (PaNa), and PBS. Quantitative graphs showing the proportion of late apoptotic/necrotic (Annexin V⁺/PI⁺) neutrophils ( B ) and early apoptotic (Annexin V⁺/PI⁻) neutrophils ( C ) under different treatments ( n = 3). D Schematic representation of neutrophil migration in vitro for 3 h at 37 °C. Created in BioRender. Yang, Q. (2026) https://BioRender.com/fm3t1j5 . Representative images of migrated neutrophils on the lower membrane stained with DAPI and photographed using the multifunctional enzyme reader. E Numbers of migrating neutrophils ( n = 3). F Volcano plot of differential gene expression in sodium decanoate-treated and PBS-treated neutrophils. G Heatmap showing the DEGs identified via RNA-seq in the presence of decanoic acid or PBS. H Quantitative reverse transcription PCR (qRT‒PCR) validation of gene expression changes in neutrophils treated with decanoic acid, palmitic acid and PBS ( n = 4). I Cell supernatant CXCL8 expression in neutrophils treated with decanoic acid, palmitic acid and PBS, as determined by ELISA ( n = 4). J Simplified schematic illustrating the coculture assay of neutrophils with Caco-2 cells, detailing the experimental setup. Created in BioRender. Yang, Q. (2026) https://BioRender.com/74ys0y4 . K qRT‒PCR validation of gene expression changes in Caco-2 cells after coculture with different fatty-acid-treated neutrophils ( n = 3). Data are shown as mean ± SEM, with representative results from at least three independent experiments. P values were determined by one-way ANOVA with Holm–Šidák test ( B , C ), one-way ANOVA with Dunnett’s test ( E , K ), one-way ANOVA with Tukey test ( H – I ). DeaNa, sodium decanoate; PaNa, sodium palmitate. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 and ns P > 0.05. Exact P values: B DeaNa vs PBS, P = 0.0465, PaNa vs PBS, P = 0.0002; C DeaNa vs PBS, P < 0.0001, PaNa vs PBS, P < 0.0001; E DeaNa vs PBS, P = 0.0342, PaNa vs PBS, P = 0.4726; H Cxcl2 , DeaNa vs PBS, P = 0.0015, PaNa vs PBS, P = 0.0001; Cxcl8 , DeaNa vs PBS, P = 0.0120, PaNa vs PBS, P = 0.0725; Cpt1a , DeaNa vs PBS, P = 0.0441, PaNa vs PBS, P < 0.0001; Osm , DeaNa vs PBS, P = 0.0078, PaNa vs PBS, P = 0.0019; Vegfa , DeaNa vs PBS, P = 0.0319, PaNa vs PBS, P = 0.0009. I DeaNa vs PBS, P = 0.0016, PaNa vs PBS, P = 0.0101. K CXCL8, DeaNa vs PBS, P < 0.0001, PaNa vs PBS, P = 0.9138; OSM, DeaNa vs PBS, P = 0.0865, PaNa vs PBS, P = 0.0031; VEGFA, DeaNa vs PBS, P = 0.0053, PaNa vs PBS, P < 0.0001; AREG, DeaNa vs PBS, P = 0.0451, PaNa vs PBS, P = 0.0391; PTGS2, DeaNa vs PBS, P = 0.0015, PaNa vs PBS, P = 0.0224.

    Journal: Nature Communications

    Article Title: Fusobacterium periodonticum promotes colorectal tumorigenesis via decanoic acid-driven neutrophil chemotaxis

    doi: 10.1038/s41467-026-74591-y

    Figure Lengend Snippet: A Flow cytometry analysis of neutrophil apoptosis after coculture with sodium decanoate (DeaNa), sodium palmitate (PaNa), and PBS. Quantitative graphs showing the proportion of late apoptotic/necrotic (Annexin V⁺/PI⁺) neutrophils ( B ) and early apoptotic (Annexin V⁺/PI⁻) neutrophils ( C ) under different treatments ( n = 3). D Schematic representation of neutrophil migration in vitro for 3 h at 37 °C. Created in BioRender. Yang, Q. (2026) https://BioRender.com/fm3t1j5 . Representative images of migrated neutrophils on the lower membrane stained with DAPI and photographed using the multifunctional enzyme reader. E Numbers of migrating neutrophils ( n = 3). F Volcano plot of differential gene expression in sodium decanoate-treated and PBS-treated neutrophils. G Heatmap showing the DEGs identified via RNA-seq in the presence of decanoic acid or PBS. H Quantitative reverse transcription PCR (qRT‒PCR) validation of gene expression changes in neutrophils treated with decanoic acid, palmitic acid and PBS ( n = 4). I Cell supernatant CXCL8 expression in neutrophils treated with decanoic acid, palmitic acid and PBS, as determined by ELISA ( n = 4). J Simplified schematic illustrating the coculture assay of neutrophils with Caco-2 cells, detailing the experimental setup. Created in BioRender. Yang, Q. (2026) https://BioRender.com/74ys0y4 . K qRT‒PCR validation of gene expression changes in Caco-2 cells after coculture with different fatty-acid-treated neutrophils ( n = 3). Data are shown as mean ± SEM, with representative results from at least three independent experiments. P values were determined by one-way ANOVA with Holm–Šidák test ( B , C ), one-way ANOVA with Dunnett’s test ( E , K ), one-way ANOVA with Tukey test ( H – I ). DeaNa, sodium decanoate; PaNa, sodium palmitate. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 and ns P > 0.05. Exact P values: B DeaNa vs PBS, P = 0.0465, PaNa vs PBS, P = 0.0002; C DeaNa vs PBS, P < 0.0001, PaNa vs PBS, P < 0.0001; E DeaNa vs PBS, P = 0.0342, PaNa vs PBS, P = 0.4726; H Cxcl2 , DeaNa vs PBS, P = 0.0015, PaNa vs PBS, P = 0.0001; Cxcl8 , DeaNa vs PBS, P = 0.0120, PaNa vs PBS, P = 0.0725; Cpt1a , DeaNa vs PBS, P = 0.0441, PaNa vs PBS, P < 0.0001; Osm , DeaNa vs PBS, P = 0.0078, PaNa vs PBS, P = 0.0019; Vegfa , DeaNa vs PBS, P = 0.0319, PaNa vs PBS, P = 0.0009. I DeaNa vs PBS, P = 0.0016, PaNa vs PBS, P = 0.0101. K CXCL8, DeaNa vs PBS, P < 0.0001, PaNa vs PBS, P = 0.9138; OSM, DeaNa vs PBS, P = 0.0865, PaNa vs PBS, P = 0.0031; VEGFA, DeaNa vs PBS, P = 0.0053, PaNa vs PBS, P < 0.0001; AREG, DeaNa vs PBS, P = 0.0451, PaNa vs PBS, P = 0.0391; PTGS2, DeaNa vs PBS, P = 0.0015, PaNa vs PBS, P = 0.0224.

    Article Snippet: After treatment, cells were collected and stained using an Annexin V-FITC/Propidium Iodide (PI)-PE Apoptosis Detection Kit (MedChemExpress, USA).

    Techniques: Flow Cytometry, Migration, In Vitro, Membrane, Staining, Gene Expression, RNA Sequencing, Reverse Transcription, Biomarker Discovery, Expressing, Enzyme-linked Immunosorbent Assay, Co-culture Assay